The genome is folded into a hierarchical structure butnot arranged in a linear sequence on the chromosomes within the nucleus. Spatial genomics, i.e., Three-dimensional (3D) genomics is an emerging discipline for studying the three-dimensionalstructure of the genome, and its effects on the biological processes such asDNA replication and recombination, and gene expression regulation. The goal ofthis course is to help students understand the genome organization in three-dimensional,by introducing the relevant techniques of Spatial genomics and their applications.Through the study of this course, students will be able to master theapplication of three-dimensional genomics techniques and methods to study the genelong-range regulation mechanisms of chromatin conformation. This course adoptsthe teaching method of combining "theory teaching" and"scientific case analysis" to help students understand the coursecontent, exercise their scientific research thinking ability, and pave a road forcultivating high-level innovative talents in the field of Spatial (3D) genomics.