Frontiersin Biophysics is a course that introduces how to apply the laws and methods ofphysics to biological research. The goal is to expand students' vision ofbiophysical research and deepen their understanding of the functions ofbiological macromolecules and their regulation through quantitative analysis oflife processes and the development of simple biophysical models, thus helpingstudents to build a better knowledge system and solidify the foundations ofscientific research. We will start by introducing some facts to gain aquantitative understanding of biological phenomena. Next, we will examine thephysical and chemical properties of biological membranes, and the energy transferand movement characteristics of biological systems. Then, we will focus on aseries of techniques that have driven advances in studying the structure andfunction of proteins and nucleic acids. Finally, we will introduce proteinfolding mechanisms, review advances in the study of amyloid filaments andprotein assemblies driven by liquid-liquid phase separation.