Entering the post-genome era, the rapid advancement in functional genomics have invented multiple cutting-edge omics tools to investigate the human diseases and the therapeutic processes in a systematic way meanwhile disclosing dramatic variations among individual patients. The importance of fulfilling personalized medicine, also namely Precision Medicine （PM）, has been widely recognized, albeit possessing both opportunities and challenges.This course aims to deliver two main modules/parts in PM: the omics tools in functional genomics, and their applications in PM with the focus on Pharmacogenomics. Module-1 “Functional genomics, systems biology, and omics technologies” covers the basic concept and scopes of human genome, key molecular terminologies in functional genomics (from genome to transcriptome, proteome, and metabolome); the history, concept, technique, and applications of cutting-edge omics tools including next-generation sequencing (2nd & 3rd generation) for genome/transcriptome analyses, as well as technologies in proteomics and metabolomics. The most recent development on single-cell technologies, including spatially resolved single-cell technologies are also be covered. The analytic tools are briefly introduced with the focuses on the result interpretation and analyses of each omics tool.Module-2 “Precision Medicine (PM) and Omics” covers the basic concept, goals, and approaches of PM with particular focus on Pharmacogenomics, which discovers genetic/genomic and non-genetic variations critical to drug sensitivity and metabolism for individual patient. The key omics tools and analytical methods introduced in Module-1 are also discussed for pharmacogenomic studies. Moreover, the ethics to use omics tools and management of pharmacogenomic data are also discussed in the course. Within this course, the students are expected to get familiar to basic concepts and knowledge in functional genomics and relevant cutting-edge omics tools at transcriptomic/proteomic/metabolomic level to study genetic/non-genetics variations responsible for disease development and therapeutic responses. Through the course learning, the students are expected to grasp the knowledge on the status, perspective, opportunities and challenges, as well as ethics of pharmacogenomics and precision medicine, and also to deliver their own opinions.