Single-molecule techniques is a course thatintroduces the development of cutting-edge single-molecule techniques and theirapplications in solving complicated biological questions. Advances intechnologies are essential for life sciences, therefore this course aims toexpand the knowledgeof versatile single-molecule techniques and their unique advantages in biologicalresearches for students. In combination with fundamental biological questions,the course will deepen the understanding and critical thinking of dynamicallyregulated biological processes, helping them to explore important questionswith new angle and methods.