Pathogens, including viruses, bacteria,fungi, and parasites, are a major threat to human health. Diagnosis ofpathogens is a prerequisite for their effective prevention, control, andtreatment. The course “Frontiers in Pathogen DiagnosticTechniques” is intended for graduate students in public health, biomedicalsciences, biology, and clinical medicine who need the knowledge of pathogendiagnosis. This course include 48 credit hours, specifically covering the basiccharacteristics of pathogens and their diagnostic markers, sample processingand isolation and culture of pathogens, diagnostic imaging techniques of pathogens,diagnostic techniques of nucleic acids, antibodies, antigens, and metabolites,diagnostic techniques of mutations, large-scale screening and rapid diagnostictechniques, diagnostic techniques of surveillance and unknown pathogens, theapplication of microfluidics, microarrays, biosensors, mass spectrometry,artificial intelligence in pathogen diagnosis, and case studies. The course isintended to use a combination of lectures and seminars to promote the motivationand participation through a variety of teaching methods. This course will notonly help to expand students' professional knowledge in pathogen diagnosis, butalso improve students' scientific research performance and career development.