Molecular Biology is a course is about genes - their structure and function- at the molecular level. The objective is to give you a firm and rigorous foundation in understanding gene expression and its regulation. We will begin with a review of structure of protein and nucleic acid, and the physical and chemical properties that drive interactions of protein with nucleic acid. Then we will study methods and technologies applied to study genes. Next, we will study the molecular mechanics of DNA replication, DNA damage repair, transcription in both prokaryotic and eukaryotic organisms, with an emphasis on how the activity of genes is regulated at the molecular level through nucleic acid-protein interactions. Lastly, genomics and systems biology will also be introduced and we will review advances in genomics projects that are altering our understanding of molecular biology.