This course emphasizes on the separation and analysis of complex analytes, and instruments covered in this course should reflect new advances in the field of Instrumental Analysis. Experiments in this course should address the basic principles of the instruments and help the students to understand the fundamental concepts and mechanisms of the instruments. This course should facilitate the instructor to cover lab processes from both theoretical and operational perspectives, and let the students complete the experimental processes. The chosen instruments should be meet the needs of majority of today’s undergraduate chemistry and other multi-disciplinary (e.g. material science, environmental science) programs.